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Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

Ken-ichiro Kamei,*abcde   Minori Ohashi,abcde   Eric Gschweng,ef   Quinn Ho,abcdeg   Jane Suh,abcde   Jinghua Tang,e   Zeta Tak For Yu,abcde   Amander T. Clark,eh   April D. Pyle,ef   Michael A. Teitell,ei   Ki-Bum. Lee,j   Owen N. Witteacefk and   Hsian-Rong Tseng*abcde  
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Corresponding authors
a
Department of Molecular & Medical Pharmacology, University of California, Los Angeles, USA
E-mail: kkamei@mednet.ucla.edu, hrtseng@mednet.ucla.edu
b
Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California, Los Angeles, USA
c
California NanoSystems Institute, University of California, Los Angeles, USA
d
Institute for Molecular Medicine, University of California, Los Angeles, USA
e
Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, USA
f
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, USA
g
Department of Biological Chemistry, University of California, Los Angeles, USA
h
Department of Molecular Cell and Developmental Biology, Los Angeles, USA
i
Department of Pathology and Laboratory Medicine, Los Angeles, USA
j
Department of Chemistry & Chemical Biology, Institute for Advanced Materials, Devices and Nanotechnology, Rutgers Stem Cell Research Center, Rutgers, The State University of New Jersey, Piscataway, USA
k
Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, USA
Lab Chip, 2010,10, 1113-1119

DOI: 10.1039/B922884E
Received 04 Nov 2009, Accepted 11 Feb 2010
First published online 16 Mar 2010
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