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Issue 7, 2010
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A microfluidic chip-compatible bioassay based on single-molecule detection with high sensitivity and multiplexing

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Abstract

Many applications in pharmaceutical development, clinical diagnostics, and biological research demand rapid detection of multiple analytes (multiplexed detection) in a minimal volume. This need has led to the development of several novel array-based sensors. The most successful of these so far have been suspension arrays based on polystyrene beads. However, the 5 µm beads used for these assays are incompatible with most microfluidic chip technologies, mostly due to clogging problems. The challenge, then, is to design a detection particle that has high information content (for multiplexed detection), is compatible with miniaturization, and can be manufactured easily at low cost. DNA is a solid molecular wire that is easily produced and manipulated, which makes it a useful material for nanoparticles. DNA molecules are very information-rich, readily deformable, and easily propagated. We exploit these attributes in a suspension array sensor built from specialized recombinant DNA, Digital DNA, that carries both specific analyte-recognition units, and a geometrically encoded identification pattern. Here we show that this sensor combines high multiplexing with high sensitivity, is biocompatible, and has sufficiently small particle size to be used within microfluidic chips that are only 1 µm deep. We expect this technology will be the foundation of a broadly applicable technique to identify and quantitate proteins, nucleic acids, viruses, and toxins simultaneously in a minimal volume.

Graphical abstract: A microfluidic chip-compatible bioassay based on single-molecule detection with high sensitivity and multiplexing

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Publication details

The article was received on 23 Oct 2009, accepted on 11 Dec 2009 and first published on 14 Jan 2010


Article type: Paper
DOI: 10.1039/B922106A
Citation: Lab Chip, 2010,10, 843-851
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    A microfluidic chip-compatible bioassay based on single-molecule detection with high sensitivity and multiplexing

    R. E. Burton, E. J. White, T. R. Foss, K. M. Phillips, R. H. Meltzer, N. Kojanian, L. W. Kwok, A. Lim, N. L. Pellerin, N. V. Mamaeva and R. Gilmanshin, Lab Chip, 2010, 10, 843
    DOI: 10.1039/B922106A

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