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Issue 9, 2010
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DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device

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Abstract

We report the proof of concept of a novel DNA sequencing technology called sequencing by denaturation (SBD). SBD is based on the Sanger sequencing reaction performed on amplified target templates immobilized on a solid surface followed by the denaturation of these Sanger fragments. As these fluorescently labeled fragments denature sequentially, the fluorescence intensities in the four channels corresponding to the four base types are monitored in a flow cell. A sequencing instrument with a microfluidic flowcell has been custom-designed to integrate automated fluidics, temperature control, and fluorescence imaging. The denaturation profiles of several synthetic oligonucleotides were measured with this system and our data demonstrated the ability to sequence short DNA by SBD. SBD is a simple and fast method with the potential to improve the speed and cost of large-scale genome re-sequencing.

Graphical abstract: DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device

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Publication details

The article was received on 16 Oct 2009, accepted on 08 Jan 2010 and first published on 09 Feb 2010


Article type: Paper
DOI: 10.1039/B921417H
Citation: Lab Chip, 2010,10, 1153-1159
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    DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device

    Y. Chen, E. E. Roller and X. Huang, Lab Chip, 2010, 10, 1153
    DOI: 10.1039/B921417H

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