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Issue 4, 2010
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Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (μFIC)

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Abstract

Microfluidic systems have significant implications in the field of in vitro cell-based assays since they may allow conventional cell-based assays to be conducted in an automated and high-throughput fashion. In this study, we combined a simple microfluidic cells-on-chip system with a morphology-based image cytometric analysis approach for the assessment of Cd2+ induced apoptosis of Chang liver cell line. A simple and efficient in situ monitoring method for quantifying the progress of a cell death event was developed and is presented here. Reasonable agreement of the estimated EC50 value from this study with those from the literature and a close correlation between the observed changes in cell morphology (i.e., circularity) and the amount of reactive oxygen species (ROS) generation confirmed the validity of this morphology-based microfluidic image cytometric (μFIC) assessment method. We propose this morphology-based μFIC approach as an easy and efficient way to assess cytotoxicity which can be adapted to high-throughput screening platforms for in vitro cytotoxicity assays as well as drug screening.

Graphical abstract: Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (μFIC)

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Publication details

The article was received on 06 Oct 2009, accepted on 10 Dec 2009 and first published on 12 Jan 2010


Article type: Communication
DOI: 10.1039/B920890A
Citation: Lab Chip, 2010,10, 415-417
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    Morphology-based assessment of Cd2+ cytotoxicity using microfluidic image cytometry (μFIC)

    M. J. Kim, K. H. Lim, H. J. Yoo, S. W. Rhee and T. H. Yoon, Lab Chip, 2010, 10, 415
    DOI: 10.1039/B920890A

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