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Issue 8, 2010
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Infrared light induced patterning of proteins on ppNIPAM thermoresponsive thin films: a “protein laser printer”

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Abstract

Protein micropatterns have applications in fundamental life sciences and clinical medicine. In this work, we present a new technique to create 2-D protein micropatterns by local activation of a thin film of thermoresponsive plasma-deposited poly(N-isopropylacrylamide) (ppNIPAM) using a computer-controlled infrared laser beam. While the whole substrate is exposed to the protein solution, protein deposition happens only at laser-activated locations. A few seconds of laser exposure is all that is required to form a pattern with resolution in the single micrometre range. Successful ligand binding after protein deposition indicates that protein function remains intact after laser-induced adsorption onto ppNIPAM. This rapid, simple technique advances currently available strategies for protein patterning by its potential to pattern proteins in an enclosed environment or onto a 3-D scaffold.

Graphical abstract: Infrared light induced patterning of proteins on ppNIPAM thermoresponsive thin films: a “protein laser printer”

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Publication details

The article was received on 06 Oct 2009, accepted on 16 Dec 2009 and first published on 20 Jan 2010


Article type: Paper
DOI: 10.1039/B920883F
Citation: Lab Chip, 2010,10, 1079-1085
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    Infrared light induced patterning of proteins on ppNIPAM thermoresponsive thin films: a “protein laser printer”

    X. Cheng, E. Yegan Erdem, S. Takeuchi, H. Fujita, B. D. Ratner and K. F. Böhringer, Lab Chip, 2010, 10, 1079
    DOI: 10.1039/B920883F

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