Jump to main content
Jump to site search

Issue 4, 2010
Previous Article Next Article

Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

Author affiliations

Abstract

Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95% removal efficiency at 0.2–0.5 mm s−1 with 100% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells.

Graphical abstract: Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Oct 2009, accepted on 14 Dec 2009 and first published on 19 Jan 2010


Article type: Paper
DOI: 10.1039/B920590J
Citation: Lab Chip, 2010,10, 438-445
  •   Request permissions

    Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

    H. Shafiee, M. B. Sano, E. A. Henslee, J. L. Caldwell and R. V. Davalos, Lab Chip, 2010, 10, 438
    DOI: 10.1039/B920590J

Search articles by author

Spotlight

Advertisements