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Issue 6, 2010
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A digital microfluidic platform for the automation of quantitative biomolecular assays

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Abstract

A digital microfluidic platform for the automation of quantitative, multi-step biomolecular assays is developed and optimized. The platform consists of a 2-dimensional array of microvalves that can be programmed to perform reagent routing, mixing, rinsing, serial dilution, and many other operations using nanolitre scale volumes of sample. Discrete transfer of fluid between microvalves is characterized using gravimetric flow analysis and optimized to achieve maximum efficiency. Protocols for on-chip reagent mixing and serial dilution are optimized to achieve linearity over a 1000-fold dilution range. These optimized programs are used to develop a rapid, quantitative assay for hydrogen peroxide, a biomarker of oxidative stress. A sub-micromolar limit of detection is demonstrated with an 8.5 min program runtime, thus establishing this platform as an effective tool for the automation of multi-step bioassays. The programmability of this system enables rapid development of diverse assay protocols on a common chip format.

Graphical abstract: A digital microfluidic platform for the automation of quantitative biomolecular assays

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Publication details

The article was received on 01 Oct 2009, accepted on 23 Nov 2009 and first published on 23 Dec 2009


Article type: Paper
DOI: 10.1039/B920124F
Citation: Lab Chip, 2010,10, 685-691
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    A digital microfluidic platform for the automation of quantitative biomolecular assays

    E. C. Jensen, B. P. Bhat and R. A. Mathies, Lab Chip, 2010, 10, 685
    DOI: 10.1039/B920124F

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