Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 6, 2010
Previous Article Next Article

Circular compartmentalized microfluidic platform: Study of axon–glia interactions

Author affiliations

Abstract

We describe a compartmentalized circular microfluidic platform that enables directed cell placement within defined microenvironments for the study of axon–glia interactions. The multi-compartment platform consists of independent units of radial microchannel arrays that fluidically isolate somal from axonal compartments. Fluidic access ports punched near the microchannels allow for direct pipetting of cells into the device. Adjacent somal or axonal compartments can be readily merged so that independent groups of neurons or axons can be maintained in either separate or uniform microenvironments. We demonstrate three distinct modes of directed cell placement in this device, to suit varying experimental needs for the study of axon–glia interactions: (1) centrifugation of the circular platform can result in a two-fold increase in axonal throughput in microchannels and provides a new technique to establish axon–glia interactions; (2) microstencils can be utilized to directly place glial cells within areas of interest; and (3) intimate axon–glia co-culture can be attained via standard pipetting techniques. We take advantage of this microfluidic platform to demonstrate a two-fold preferential accumulation of microglia specifically near injured CNS axons, an event implicated in the maintenance and progression of a number of chronic neuroinflammatory and neurodegenerative diseases.

Graphical abstract: Circular compartmentalized microfluidic platform: Study of axon–glia interactions

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 08 Sep 2009, accepted on 27 Nov 2009 and first published on 05 Jan 2010


Article type: Paper
DOI: 10.1039/B918640A
Citation: Lab Chip, 2010,10, 741-747
  •   Request permissions

    Circular compartmentalized microfluidic platform: Study of axon–glia interactions

    S. Hosmane, I. H. Yang, A. Ruffin, N. Thakor and A. Venkatesan, Lab Chip, 2010, 10, 741
    DOI: 10.1039/B918640A

Search articles by author