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Issue 22, 2010
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Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients

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Abstract

Endothelial cell (EC) sprouting morphogenesis is a critical step during angiogenesis, the formation of new blood vessels from existing conduits. Here, three-dimensional sprouting morphogenesis was examined using in vitro microfluidic devices that enabled the separate and simultaneous tuning of biomechanical and soluble biochemical stimuli. Quantitative analysis of endothelial sprout formation demonstrated that the ability of vascular endothelial growth factor (VEGF) to regulate stable sprout formation was mediated by the density of the surrounding collagen/fibronectin matrix. The coordinated migration and proliferation of multiple ECs to form stable sprouts were enhanced at intermediate matrix densities (1.2–1.9 mg ml−1), while lower densities resulted in uncoordinated migration (0.3–0.7 mg ml−1) and higher densities resulted in broad cell clusters that did not elongate (2.7 mg ml−1). Within the permissive range of matrix biomechanics, higher density matrices resulted in shorter, thicker, and slower-growing sprouts. The sprouts in higher density matrices also were more likely to polarize towards higher VEGF concentrations, included more cells per cross-sectional area, and demonstrated more stable lumen formation compared to sprouts in lower density matrices. These results quantitatively demonstrate that matrix density mediates VEGF-induced sprout polarization and lumen formation, potentially by regulating the balance between EC migration rate and proliferation rate.

Graphical abstract: Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients

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Publication details

The article was received on 13 Apr 2010, accepted on 26 Jul 2010 and first published on 01 Sep 2010


Article type: Paper
DOI: 10.1039/C005069E
Citation: Lab Chip, 2010,10, 3061-3068
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    Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients

    A. Shamloo and S. C. Heilshorn, Lab Chip, 2010, 10, 3061
    DOI: 10.1039/C005069E

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