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Issue 2, 2010
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Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media

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Abstract

Optoelectronic tweezers (OET), based on light-induced dielectrophoresis, has been shown as a versatile tool for parallel manipulation of micro-particles and cells (P. Y. Chiou, A. T. Ohta and M. C. Wu, Nature, 2005, 436, 370–372).1 However, the conventional OET device cannot operate in cell culture media or other high-conductivity physiological buffers due to the limited photoconductivity of amorphous silicon. In this paper, we report a new phototransistor-based OET (Ph-OET). Consisting of single-crystalline bipolar junction transistors, the Ph-OET has more than 500× higher photoconductivity than amorphous silicon. Efficient cell trapping of live HeLa and Jurkat cells in Phosphate Buffered Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) has been demonstrated using a digital light projector, with a cell transport speed of 33 µm/sec, indicating a force of 14.5 pN. Optical concentration of cells and real-time control of individually addressable cell arrays have also been realized. Precise control of separation between two cells has also been demonstrated. We envision a new platform for single cell studies using Ph-OET.

Graphical abstract: Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media

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Publication details

The article was received on 14 Apr 2009, accepted on 05 Aug 2009 and first published on 07 Sep 2009


Article type: Paper
DOI: 10.1039/B906593H
Citation: Lab Chip, 2010,10, 165-172
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    Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media

    H. Hsu, A. T. Ohta, P. Chiou, A. Jamshidi, S. L. Neale and M. C. Wu, Lab Chip, 2010, 10, 165
    DOI: 10.1039/B906593H

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