Issue 2, 2010

Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media

Abstract

Optoelectronic tweezers (OET), based on light-induced dielectrophoresis, has been shown as a versatile tool for parallel manipulation of micro-particles and cells (P. Y. Chiou, A. T. Ohta and M. C. Wu, Nature, 2005, 436, 370–372).1 However, the conventional OET device cannot operate in cell culture media or other high-conductivity physiological buffers due to the limited photoconductivity of amorphous silicon. In this paper, we report a new phototransistor-based OET (Ph-OET). Consisting of single-crystalline bipolar junction transistors, the Ph-OET has more than 500× higher photoconductivity than amorphous silicon. Efficient cell trapping of live HeLa and Jurkat cells in Phosphate Buffered Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) has been demonstrated using a digital light projector, with a cell transport speed of 33 µm/sec, indicating a force of 14.5 pN. Optical concentration of cells and real-time control of individually addressable cell arrays have also been realized. Precise control of separation between two cells has also been demonstrated. We envision a new platform for single cell studies using Ph-OET.

Graphical abstract: Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media

Supplementary files

Article information

Article type
Paper
Submitted
14 Apr 2009
Accepted
05 Aug 2009
First published
07 Sep 2009

Lab Chip, 2010,10, 165-172

Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media

H. Hsu, A. T. Ohta, P. Chiou, A. Jamshidi, S. L. Neale and M. C. Wu, Lab Chip, 2010, 10, 165 DOI: 10.1039/B906593H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements