The influence of proteins on the dispersability and cell-biological activity of silver nanoparticles

S. Kittler; C. Greulich; J. S. Gebauer; J. Diendorf; L. Treuel; L. Ruiz; J. M. Gonzalez-Calbet; M. Vallet-Regi; R. Zellner; M. Köller; M. Epple

doi:10.1039/B914875B

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Journal of Materials Chemistry

Issue 3, 2010

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The influence of proteins on the dispersability and cell-biological activity of silver nanoparticles

S. Kittler , C. Greulich , J. S. Gebauer , J. Diendorf , L. Treuel , L. Ruiz , J. M. Gonzalez-Calbet , M. Vallet-Regi , R. Zellner , M. Köller and M. Epple

J. Mater. Chem., 2010, 20, 512-518

DOI: 10.1039/B914875B
Received 22 Jul 2009, Accepted 19 Oct 2009
First published on the web 11 Nov 2009

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Spherical silver nanoparticles with a diameter of 50 ± 20 nm and stabilized with either poly(N-vinylpyrrolidone) (PVP) or citrate were dispersed in different cell culture media: (i) pure RPMI, (ii) RPMI containing up to 10% of bovine serum albumin (BSA), and (iii) RPMI containing up to 10% of fetal calf serum (FCS). The agglomeration behavior of the nanoparticles was studied with dynamic light scattering and optical microscopy of individually tracked single particles. Whereas strong agglomeration was observed in pure RPMI and in the RPMI–BSA mixture within a few hours, the particles remained well dispersed in RPMI–FCS. In addition, the biological effect of PVP-stabilized silver nanoparticles and of silver ions on human mesenchymal stem cells (hMSCs) was studied in pure RPMI and also in RPMI–BSA and RPMI–FCS mixtures, respectively. Both proteins considerably increased the cell viability in the presence of silver ions and as well as silver nanoparticles, indicating a binding of silver by these proteins.

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Journal of Materials Chemistry

The influence of proteins on the dispersability and cell-biological activity of silver nanoparticles

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