Issue 6, 2010

Calcium phosphate/PLGA-mPEG hybrid porous nanospheres: A promising vector with ultrahigh gene loading and transfection efficiency

Abstract

A facile room-temperature method for the fabrication of calcium phosphate (CaP)/PLGA-mPEG hybrid porous nanospheres has been developed. The hybrid CaP/PLGA-mPEG nanospheres obtained were 20∼60 nm in diameter and exhibited porous structure, and they were applied as DNA vectors for DNA loading and in vitro transfection. The results showed that the DNA binding capacity and transfection efficiency of the as-prepared hybrid porous nanospheres were much higher than those of the CaP precipitates prepared according to the standard CaP transfection procedure and mesoporous silica reported before. MTT assays confirmed that the CaP/PLGA-mPEG hybrid porous nanospheres were quite safe. In addition, both CaP and PLGA-mPEG are biocompatible and biodegradable, thus the as-prepared CaP/PLGA-mPEG hybrid porous nanospheres are promising in gene delivery.

Graphical abstract: Calcium phosphate/PLGA-mPEG hybrid porous nanospheres: A promising vector with ultrahigh gene loading and transfection efficiency

Article information

Article type
Paper
Submitted
24 Aug 2009
Accepted
12 Nov 2009
First published
15 Dec 2009

J. Mater. Chem., 2010,20, 1161-1166

Calcium phosphate/PLGA-mPEG hybrid porous nanospheres: A promising vector with ultrahigh gene loading and transfection efficiency

K. Wang, L. Zhou, Y. Sun, G. Wu, H. Gu, Y. Duan, F. Chen and Y. Zhu, J. Mater. Chem., 2010, 20, 1161 DOI: 10.1039/B917441A

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