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Issue 6, 2010
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D-(−)-Lactic acid production from cellobiose and cellulose by Lactobacillus lactis mutant RM2-24

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Abstract

Lactobacillus lactis mutant RM2-24 utilizes cellobiose efficiently, converting it into D-(−)-lactic acid. Cellobiose-degrading enzyme activities were determined for whole cells, cell extracts and disrupted cells. Aryl-β-glucosidase activity was detected in whole cells and disrupted cells, suggesting that these activities are confined to the cells. The mutant produced 80 g l−1 of lactic acid from 100 g l−1 of cellobiose with 1.66 g l−1 h−1 productivity. Production of D-lactic acid from different cellulose samples was also studied. The cellulose samples at high concentration (10%) were hydrolyzed by cellulase enzyme preparation (10 FPU g−1 cellulose) derived from Penicillium janthinellum mutant EU1 generated in our own laboratory. We obtained a maximum 72% hydrolysis, yielding glucose and cellobiose as the main end products. Lactic acid was produced from these cellulose samples by simultaneous saccharification and fermentation (SSF) in a media containing a cellulase enzyme preparation derived from Penicillium janthinellum mutant EU1 and cellobiose utilizing Lactobacillus lactis mutant RM2-24. A maximum lactic acid concentration of 73 g l−1 was produced from a concentration of 100 g l−1 of bagasse-derived cellulose, the highest productivity and yield being 1.52 g l−1 h−1 and 0.73 g g−1, respectively. Considering that bagasse is a waste material available in abundance, we propose to use this biomass to produce cellulose and then sugars, which can be fermented to valuable products such as ethanol and lactic acid.

Graphical abstract: d-(−)-Lactic acid production from cellobiose and cellulose by Lactobacillus lactis mutant RM2-24

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Publication details

The article was received on 09 Dec 2009, accepted on 01 Apr 2010 and first published on 14 May 2010


Article type: Paper
DOI: 10.1039/B925975A
Citation: Green Chem., 2010,12, 1106-1109
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    D-(−)-Lactic acid production from cellobiose and cellulose by Lactobacillus lactis mutant RM2-24

    M. Singhvi, D. Joshi, M. Adsul, A. Varma and D. Gokhale, Green Chem., 2010, 12, 1106
    DOI: 10.1039/B925975A

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