Specific cellular water dynamics observed in vivo by neutron scattering and NMR

Marion Jasnin; Andreas Stadler; Moeava Tehei; Giuseppe Zaccai

doi:10.1039/C0CP01048K

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Physical Chemistry Chemical Physics

Issue 35, 2010

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Specific cellular water dynamics observed in vivo by neutron scattering and NMR

Marion Jasnin,^a Andreas Stadler,^b Moeava Tehei^c^d and Giuseppe Zaccai*^e

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Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, D-82152 Martinsried, Germany

Institute of Structural Biology and Biophysics, ISB-2: Molecular Biophysics, Forschungszentrum Jülich, D-52425 Jülich, Germany

University of Wollongong, School of Chemistry and Centre for Medical Bioscience, Wollongong, Australia

Australian Institute of Nuclear Science and Engineering, Menai, Australia

Institut Laue Langevin, 6 rue Jules Horowitz, BP 156, 38042 Grenoble Cedex 9, France
E-mail: zaccai@ill.fr

Phys. Chem. Chem. Phys., 2010,12, 10154-10160

DOI: 10.1039/C0CP01048K
Received 01 Jul 2010, Accepted 04 Aug 2010
First published online 16 Aug 2010

This article is part of themed collection: Water in biological systems

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Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length ( [similar] ångstrøm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not “tamed” by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration water an intracellular water component was observed with significantly slowed down translational diffusion. The results are discussed and compared to observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMR—a method that is sensitive to water rotational dynamics on a wide range of time scales.