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Issue 41, 2010
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Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides

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Abstract

Previously we have shown that the presence of 3-nitrotyrosine within a peptide sequence severely depletes the peptide backbone fragments typically observed following electron capture dissociation (ECD) mass spectrometry. Instead, ECD of nitrated peptides is characterised by abundant losses of small neutrals (hydroxyl radicals, water and ammonia). Here, we investigate the origin of ammonia loss by comparing the ECD behaviour of lysine- and arginine-containing nitrated peptides, and their N-acetylated counterparts, and nitrated peptides containing no basic amino acid residues. The results reveal that ammonia loss derives from the N-terminus of the peptides, however, the key finding of this work is the insight provided into the hierarchy of various proposed ECD mechanisms: the Utah-Washington mechanism, the electron predator mechanism and the Oslo mechanism.

Graphical abstract: Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides

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Publication details

The article was received on 19 May 2010, accepted on 05 Aug 2010 and first published on 08 Sep 2010


Article type: Paper
DOI: 10.1039/C0CP00623H
Citation: Phys. Chem. Chem. Phys., 2010,12, 13394-13399
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    Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides

    A. W. Jones and H. J. Cooper, Phys. Chem. Chem. Phys., 2010, 12, 13394
    DOI: 10.1039/C0CP00623H

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