Jump to main content
Jump to site search

Issue 34, 2010
Previous Article Next Article

In situ electrochemical monitoring of reactive oxygen and nitrogen species released by single MG63 osteosarcoma cell submitted to a mechanical stress

Author affiliations

Abstract

The oxidative stress responses of single MG63 osteosarcoma cells submitted to a brief mechanical stress have been investigated by amperometry at platinized carbon fiber electrodes for monitoring and characterizing the nature and the amounts of the various reactive oxygen (ROS) and reactive nitrogen species (RNS) released. It was thus shown that, on average, a single MG63 cell released prominent amounts of reactive nitrogen species (17 fmol NO˙, 6 fmol ONOO, and 5 fmol NO2) together with a comparatively small quantity of H2O2 (2 fmol). These species resulted from the primary production of 13 fmol for O2˙ and 28 fmol for NO˙ per single cell as reconstructed from the stoichiometries of the ROS and RNS releases. The high NO˙/H2O2 and NO˙/O2˙ ratios thus found are perfectly consistent with previous claims that the malignant bone formation ability of the osteosarcoma cells is related to a specific high production of NO˙ associated to a small one of O2˙.

Graphical abstract: In situ electrochemical monitoring of reactive oxygen and nitrogen species released by single MG63 osteosarcoma cell submitted to a mechanical stress

Back to tab navigation

Publication details

The article was received on 29 Apr 2010, accepted on 03 Jun 2010 and first published on 24 Jun 2010


Article type: Paper
DOI: 10.1039/C0CP00398K
Citation: Phys. Chem. Chem. Phys., 2010,12, 10048-10054
  •   Request permissions

    In situ electrochemical monitoring of reactive oxygen and nitrogen species released by single MG63 osteosarcoma cell submitted to a mechanical stress

    R. Hu, M. Guille, S. Arbault, C. J. Lin and C. Amatore, Phys. Chem. Chem. Phys., 2010, 12, 10048
    DOI: 10.1039/C0CP00398K

Search articles by author

Spotlight

Advertisements