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Issue 14, 2010
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Guanineaspartic acid interactions probed with IR–UV resonance spectroscopy

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Abstract

Double resonance spectroscopy of clusters of guanine with aspartic acid reveals geometries similar to patterns exhibited in DNA base pairs. In the spectral region of 32 800 cm−1 to 35 500 cm−1 we observe five isomers of guanineaspartic acid clusters and assign their structures based on IR–UV hole-burning spectra and wave function theory calculations at the MP2/cc-pVDZ and MP2/cc-pVTZ levels. The calculations employed both harmonic and one-dimensional scan anharmonic approximations. Three of the isomers are similar, assigned to structures containing three hydrogen bonds and 9-enolguanine. We assign the fourth isomer to a structure containing a 9-keto tautomer of guanine and forming a triply bonded structure similar to a base pairing interaction. The fifth isomer dissociates with proton transfer upon excitation or ionization. This is the first set of experiments and high-level ab initio calculations of the isolated, microscopic interactions of an amino acid and a nucleobase, the building blocks of nucleic acids and proteins.

Graphical abstract: Guanine–aspartic acid interactions probed with IR–UV resonance spectroscopy

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Publication details

The article was received on 01 Dec 2009, accepted on 26 Feb 2010 and first published on 08 Mar 2010


Article type: Paper
DOI: 10.1039/B925340H
Citation: Phys. Chem. Chem. Phys., 2010,12, 3597-3605
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    Guanineaspartic acid interactions probed with IR–UV resonance spectroscopy

    B. O. Crews, A. Abo-Riziq, K. Pluháčková, P. Thompson, G. Hill, P. Hobza and M. S. de Vries, Phys. Chem. Chem. Phys., 2010, 12, 3597
    DOI: 10.1039/B925340H

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