Jump to main content
Jump to site search

Issue 2, 2010
Previous Article Next Article

Influence of phase separating lipids on supported lipid bilayer formation at SiO2 surfaces

Author affiliations

Abstract

The importance of the lipid phase on the formation of supported lipid bilayers (SLBs) via vesicle fusion and on the resulting SLB homogeneity at SiO2 surfaces has been studied by the quartz crystal microbalance with dissipation (QCM-D) monitoring technique. Physiologically relevant lipid compositions were chosen to correspond to different regions (ld, lo and coexistence of phases) in established phase diagrams of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), N-palmitoyl-D-erythro-sphingosylphosphorylcholine (PSM) and cholesterol. For most compositions, SLBs formed through vesicle rupture in a critical-surface-coverage dependent manner. Inclusion of PSM and cholesterol into POPC vesicles significantly impaired the vesicle rupture process such that a higher critical concentration of vesicles on the surface was needed before the rupture process started. When increasing the cholesterol content the vesicles formed SLBs containing more defects in the form of intact vesicles adsorbed on the surface up to a point (lo phase) where vesicles did not break at all but formed supported vesicular layers. The hampering of vesicle rupture is interpreted in terms of the ability of cholesterol to accommodate vesicle deformation. Experiments using elevated temperatures to alter the lipid membrane into a more fluid phase significantly improved the quality of the SLB showing the importance of both cholesterol content and the lipid phase on SLB homogeneity.

Graphical abstract: Influence of phase separating lipids on supported lipid bilayer formation at SiO2 surfaces

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 21 Jul 2009, accepted on 14 Oct 2009 and first published on 12 Nov 2009


Article type: Paper
DOI: 10.1039/B912598A
Citation: Phys. Chem. Chem. Phys., 2010,12, 453-460
  •   Request permissions

    Influence of phase separating lipids on supported lipid bilayer formation at SiO2 surfaces

    M. Sundh, S. Svedhem and D. S. Sutherland, Phys. Chem. Chem. Phys., 2010, 12, 453
    DOI: 10.1039/B912598A

Search articles by author