Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH

Arivazhagan Rajendran; Shu-ichi Nakano; Naoki Sugimoto

doi:10.1039/B922050J

Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH

Arivazhagan Rajendran,^a Shu-ichi Nakano^a^b and Naoki Sugimoto*^a^b

* Corresponding authors

^a Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
E-mail: sugimoto@konan-u.ac.jp
Fax: +81-78-303-1495
Tel: +81-78-303-1457

^b Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan

Abstract

We now present the first example in which triplet repeat DNAs adopt the i-motif structure at neutral pH by molecular crowding. Crowding stabilized the i-motif and the pK_a of N3 of cytosine was raised in such a microenvironment. Molecular crowding is known to accelerate the formation of the multi-stranded i-motif while the triplet repeats adopt the single-strand structure.

Supplementary files

Publication details

The article was received on 21 Oct 2009, accepted on 15 Dec 2009 and first published on 14 Jan 2010

Article type: Communication

DOI: 10.1039/B922050J

Citation: Chem. Commun., 2010,46, 1299-1301

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Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH

A. Rajendran, S. Nakano and N. Sugimoto, Chem. Commun., 2010, 46, 1299
DOI: 10.1039/B922050J
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Chemical Communications

Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH

Abstract

Supplementary files

Publication details

Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH

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