Issue 5, 2010
From the journal:

Chemical Communications

Soluble, folded and active subtilisin in a protic ionic liquid†

Francesco Falcioni,a   Hazel R. Housden,a   Zhenlian Ling,a   Seishi Shimizu,b   Adam J. Walkera  and  Neil C. Bruce*a  

* Corresponding authors

a CNAP, Department of Biology, University of York, York, United Kingdom
E-mail: ncb5@york.ac.uk

b York Structural Biology Laboratory, Department of Chemistry, University of York, United Kingdom

Abstract

The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.

Graphical abstract: Soluble, folded and active subtilisin in a protic ionic liquid

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Article information

DOI
https://doi.org/10.1039/B916497A
Article type
Communication
Submitted
11 Aug 2009
Accepted
11 Nov 2009
First published
26 Nov 2009

Chem. Commun., 2010,46, 749-751

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Soluble, folded and active subtilisin in a protic ionic liquid

F. Falcioni, H. R. Housden, Z. Ling, S. Shimizu, A. J. Walker and N. C. Bruce, Chem. Commun., 2010, 46, 749 DOI: 10.1039/B916497A

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