13C Labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeae

Jien Wu; Hai-Bao Zhang; Jin-Ling Xu; Russell J. Cox; Thomas J. Simpson; Lian-Hui Zhang

doi:10.1039/B916307G

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Chemical Communications

Issue 2, 2010

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¹³C Labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeae

Jien Wu , Hai-Bao Zhang , Jin-Ling Xu , Russell J. Cox , Thomas J. Simpson and Lian-Hui Zhang

Chem. Commun., 2010, 46, 333-335

DOI: 10.1039/B916307G
Received 07 Aug 2009, Accepted 30 Oct 2009
First published on the web 13 Nov 2009

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Abstract
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The structure of zeamine, a novel polyamino-amide antibiotic metabolite of Dickeya zeae has been established by NMR and detailed MS analyses; labelling studies with ¹³C-labelled acetates suggest that the repeating secondary amine containing motif may be biosynthesised via a modular PKS containing aminotransferase domains.

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Details of culture condition of D. zeae, isolation and spectroscopic data for zeamine 8
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Jien Wu
Hai-Bao Zhang
Jin-Ling Xu
Russell J. Cox
Thomas J. Simpson
Lian-Hui Zhang

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Chemical Communications

¹³C Labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeae

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