Solid-state NMR evidence for elastin-like β-turn structure in spider dragline silk

Janelle E. Jenkins; Melinda S. Creager; Emily B. Butler; Randolph V. Lewis; Jeffery L. Yarger; Gregory P. Holland

doi:10.1039/C0CC00829J

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Solid-state NMR evidence for elastin-like β-turn structure in spider dragline silk†

Janelle E. Jenkins,‡^a Melinda S. Creager,‡^b Emily B. Butler,^a Randolph V. Lewis,^b Jeffery L. Yarger*^a and Gregory P. Holland*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry and Biochemistry, Magnetic Resonance Research Center, Arizona State University, Tempe, USA
E-mail: jyarger@gmail.com, greg.holland@asu.edu
Tel: +1 480-965-0673

^b Department of Molecular Biology, University of Wyoming, Laramie, USA

Abstract

Two-dimensional homo- and heteronuclear solid-state MAS NMR experiments on ¹³C/¹⁵N-proline labeled Argiope aurantia dragline silk provide evidence for an elastin-like β-turn structure for the repetitive Gly-Pro-Gly-X-X motif prevalent in major ampullate spidroin 2 (MaSp2).

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Supplementary files

Amino acid sequences of A. aurantia silk proteins, NMR pulse sequences, ¹³C CP-MAS spectrum of ¹³C/ ¹⁵N-alanine labeled spider silk, fits to extract the percent fraction of various conformations, 2D INADEQUATE spectrum of wet silk, and table of ¹³C chemical shifts. PDF (1277K)

Article information

DOI: https://doi.org/10.1039/C0CC00829J
Article type: Communication
Submitted: 08 Apr 2010
Accepted: 20 Jul 2010
First published: 23 Aug 2010

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Chem. Commun., 2010,46, 6714-6716

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Solid-state NMR evidence for elastin-like β-turn structure in spider dragline silk

J. E. Jenkins, M. S. Creager, E. B. Butler, R. V. Lewis, J. L. Yarger and G. P. Holland, Chem. Commun., 2010, 46, 6714 DOI: 10.1039/C0CC00829J

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Chemical Communications

Solid-state NMR evidence for elastin-like β-turn structure in spider dragline silk†

Abstract

Supplementary files

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Solid-state NMR evidence for elastin-like β-turn structure in spider dragline silk

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