Issue 19, 2010
From the journal:

Chemical Communications

Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S)†

Fouzia A. Sattar,a   Daniel J. Darley,a   Francesco Politano,a   Timothy J. Woodman,a   Michael D. Threadgilla  and  Matthew D. Lloyd*a  

* Corresponding authors

a Medicinal Chemistry, Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, UK
E-mail: M.D.Lloyd@bath.ac.uk
Fax: +44 (0)1225-386114
Tel: +44 (0)1225-386786

Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) catalysed exchange of straight-chain fatty acyl-CoA α-protons. One α-proton was removed in each catalytic cycle, with the pro-S proton preferred. This reaction was most efficient for straight-chain substrates with longer side-chains. 2-Methyldecanoyl-CoA underwent α-proton exchange 3× more efficiently (as judged by Kcat/Km) than decanoyl-CoA.

Graphical abstract: Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S)

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C002509G
Article type
Communication
Submitted
05 Feb 2010
Accepted
09 Mar 2010
First published
30 Mar 2010

Chem. Commun., 2010,46, 3348-3350

Permissions

Request permissions

Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S)

F. A. Sattar, D. J. Darley, F. Politano, T. J. Woodman, M. D. Threadgill and M. D. Lloyd, Chem. Commun., 2010, 46, 3348 DOI: 10.1039/C002509G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements