Jump to main content
Jump to site search

Issue 10, 2010
Previous Article Next Article

Development of a RP-HPLC method for the simultaneous analysis of diltiazem and statin: Application in pharmaceuticals and human serum

Author affiliations

Abstract

High-performance liquid chromatographic (HPLC) method has been developed and validated for the simultaneous determination of diltiazem and statins in raw materials, their pharmaceutical formulations and human serum. In HPLC, diltiazem and statins are chromatographed using acetonitrilewater (85 : 15 v/v, pH 2.6 ± 0.02) as the mobile phase at a flow rate of 1.0 mL min−1 at ambient temperature. The separation is carried out on a Hiber®, 250-4.6 RP-18 column, equipped with a UV/visible detector at 230 nm. All the statins eluted at a different retention time and each showed good resolution from diltiazem. The method has been successfully applied to pharmaceutical formulations because no chromatographic interferences from the tablet excipients are found. The linearity is found to be in the range 0.625–20 μg mL−1. The suitability of the method for the quantitative determination of the drugs is proven by validation in accordance with the requirements laid down by the International Conference on Harmonization (ICH) guidelines. The validation results, together with statistical treatment of the data, demonstrated the reliability of this method.

Graphical abstract: Development of a RP-HPLC method for the simultaneous analysis of diltiazem and statin: Application in pharmaceuticals and human serum

Back to tab navigation

Publication details

The article was received on 24 May 2010, accepted on 27 Jun 2010 and first published on 10 Aug 2010


Article type: Paper
DOI: 10.1039/C0AY00337A
Citation: Anal. Methods, 2010,2, 1571-1576
  •   Request permissions

    Development of a RP-HPLC method for the simultaneous analysis of diltiazem and statin: Application in pharmaceuticals and human serum

    N. Sultana, M. S. Arayne, N. Shafi, F. A. Siddiqui and A. Hussain, Anal. Methods, 2010, 2, 1571
    DOI: 10.1039/C0AY00337A

Search articles by author

Spotlight

Advertisements