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Issue 8, 2010
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High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

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Abstract

In this paper we show a high-throughput method to screen the kinetics and affinity constants of many biomolecular interactions simultaneously. During the preparation of the sensor chip, ligands were serially diluted and spotted in a 6 × 4 microarray on the sensor surface. A multi-analyte sample was injected and the real-time surface plasmon resonance (SPR) responses of all the 24 microarray spots were obtained using a commercial SPR imaging instrument. The multi responses of the association and dissociation processes obtained from a single analyte injection are sufficient to calculate the rates and affinity constants of the interactions between three interactant antigen-antibody pairs using a simple monophasic kinetic model. The method drastically reduces the measurement time and cost in the benefit of increased throughput.

Graphical abstract: High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

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Publication details

The article was received on 16 Feb 2010, accepted on 27 Apr 2010 and first published on 28 May 2010


Article type: Paper
DOI: 10.1039/C0AY00112K
Citation: Anal. Methods, 2010,2, 1020-1025
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    High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

    G. Krishnamoorthy, J. Bianca Beusink and R. B. M. Schasfoort, Anal. Methods, 2010, 2, 1020
    DOI: 10.1039/C0AY00112K

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