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Issue 6, 2010
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Development of a validated HPLC-PAD-APCI/MS method for the identification and determination of iridoid glycosides in Lamiophlomis rotata

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Abstract

An HPLC-photodiode array detector (PAD)-APCI/MS method was developed for the identification of iridoid glycosides in Lamiophlomis rotata (Benth.) Kudo. The regularities of characteristic ions of iridoid glycosides obtained in APCI-MS were analyzed and used to deduce the structure of iridoid glycosides. Compared with the literature on ESI-MS, the deprotonated molecular ions were more outstanding in APCI-MS. Five major active constituents, namely, 8-O-acetylshanzhiside methyl ester, loganin, 8-deoxyshanzhiside, phloyoside II, and shanzhiside methyl ester, were simultaneously determined in ten samples by HPLC. A comprehensive validation of the method included tests of sensitivity, linearity, precision, and accuracy. The linear regressions were acquired with r > 0.999. The precision was evaluated by intra- and inter-day assays, after which relative standard deviation (R.S.D.) values were reported within 2.5%. The recovery studies for the quantified compounds were observed in the range of 95.6-101.2% with R.S.D. values less than 2.1%. The overall procedure may be suitable for the qualitative and quantitative analyses of iridoid glycosides in L. rotata and its preparations.

Graphical abstract: Development of a validated HPLC-PAD-APCI/MS method for the identification and determination of iridoid glycosides in Lamiophlomis rotata

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Publication details

The article was received on 18 Jan 2010, accepted on 19 Mar 2010 and first published on 23 Apr 2010


Article type: Paper
DOI: 10.1039/C0AY00034E
Citation: Anal. Methods, 2010,2, 714-721
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    Development of a validated HPLC-PAD-APCI/MS method for the identification and determination of iridoid glycosides in Lamiophlomis rotata

    M. Li, R. Zhang, C. Li, P. Fan, Q. Zhang and Z. Jia, Anal. Methods, 2010, 2, 714
    DOI: 10.1039/C0AY00034E

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