Issue 8, 2010

Quantification of four tobacco-specific nitrosamines in cigarette filter tips using liquid chromatography-tandem mass spectrometry

Abstract

Tobacco-specific N-nitrosamines (TSNA) have been suggested by some scientists to play an important role in tobacco smoke carcinogenesis. We have developed and validated an LC-MS/MS method for the determination of TSNA, notably N-nitrosoanabasine (NAB), N-nitrosoanatabine (NAT), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N-nitrosonornicotine (NNN), extracted from smoked cigarette filter tips. Reporting limits of 0.44, 0.89, 0.91 and 0.91ng mL−1 for NAB, NAT, NNK and NNN respectively were achieved. The newly developed method may find application in the filter analysis methodology for estimating the mouth-level exposure to NAB, NAT, NNK and NNN for cigarette smokers. TSNA levels were determined in mainstream smoke collected on industry standard Cambridge Filter Pads following smoking on a smoking machine. TSNA yields were compared with TSNA levels extracted from cigarette filter tips. However, the observation of the progressive post-smoking accumulation of TSNA during ambient storage of smoked cigarette filter tips potentially compromises use of this technique as an estimate of mouth-level exposure. Storage of smoked cigarette filter tips at sub-ambient temperatures reduced substantially the post-smoking synthesis of TSNA.

Graphical abstract: Quantification of four tobacco-specific nitrosamines in cigarette filter tips using liquid chromatography-tandem mass spectrometry

Article information

Article type
Paper
Submitted
16 Dec 2009
Accepted
28 Apr 2010
First published
04 Jun 2010

Anal. Methods, 2010,2, 1085-1094

Quantification of four tobacco-specific nitrosamines in cigarette filter tips using liquid chromatography-tandem mass spectrometry

P. M. Clayton, A. Cunningham and J. D. H. van Heemst, Anal. Methods, 2010, 2, 1085 DOI: 10.1039/B9AY00306A

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