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Issue 12, 2010
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Towards detection and identification of circulating tumour cells using Raman spectroscopy

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Abstract

Body fluids are easily accessible and contain valuable indices for medical diagnosis. Fascinating tools are tumour cells circulating in the peripheral blood of cancer patients. As these cells are extremely rare, they constitute a challenge for clinical diagnostics. In this contribution we present the Raman spectroscopic-based identification of different single cells in suspension that are found in peripheral blood of cancer patients including healthy cells like leukocytes and erythrocytes, and tumour cells like leukaemic cells and cells originating from solid tumours. Leukocytes and erythrocytes were isolated from the peripheral blood of healthy donors while myeloid leukaemia cells (OCI-AML3) and breast carcinoma derived cells (MCF-7, BT-20) were obtained from cell cultures. A laser emitting 785 nm light was used for optical trapping the single cells in the laser focus and to excite the Raman spectrum. Support vector machines were applied to develop a supervised classification model with spectra of 1210 cells originating from three different donors and three independent cultivation batches. Distinguishing tumour cells from healthy cells was achieved with a sensitivity of >99.7% and a specificity of >99.5%. In addition, the correct cell types were predicted with an accuracy of approximately 92%.

Graphical abstract: Towards detection and identification of circulating tumour cells using Raman spectroscopy

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Publication details

The article was received on 05 Aug 2010, accepted on 30 Sep 2010 and first published on 13 Oct 2010


Article type: Paper
DOI: 10.1039/C0AN00608D
Citation: Analyst, 2010,135, 3178-3182
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    Towards detection and identification of circulating tumour cells using Raman spectroscopy

    U. Neugebauer, T. Bocklitz, J. H. Clement, C. Krafft and J. Popp, Analyst, 2010, 135, 3178
    DOI: 10.1039/C0AN00608D

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