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Issue 12, 2010
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Confocal Raman microspectral imaging (CRMI) of murine stem cell colonies

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Abstract

Confocal Raman micro-spectral imaging (CRMI) is a relatively novel technique for the construction of label-free images of biological entities, such as cells or tissue sections. This method utilizes thousands of spatially resolved Raman spectra, and sophisticated image analysis algorithms, to construct images which are based strictly on the inherent biochemical abundance contrast afforded by Raman microscopy. Here, we apply this methodology to monitor the very early communication processes that occur in a growing stem cell colony, yielding information on biochemical composition during development processes of the early stages of embryogenesis.

Graphical abstract: Confocal Raman microspectral imaging (CRMI) of murine stem cell colonies

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Publication details

The article was received on 19 Jul 2010, accepted on 09 Sep 2010 and first published on 13 Oct 2010


Article type: Communication
DOI: 10.1039/C0AN00546K
Citation: Analyst, 2010,135, 3030-3033
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    Confocal Raman microspectral imaging (CRMI) of murine stem cell colonies

    E. Zuser, T. Chernenko, J. Newmark, M. Miljković and M. Diem, Analyst, 2010, 135, 3030
    DOI: 10.1039/C0AN00546K

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