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Issue 12, 2010
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Vibrational spectroscopy differentiates between multipotent and pluripotent stem cells

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Abstract

Over the last few years, there has been an increased interest in the study of stem cells in biomedicine for therapeutic use and as a source for healing diseased or injured organs/tissues. More recently, vibrational spectroscopy has been applied to study stem cell differentiation. In this study, we have used both synchrotron based FTIR and Raman microspectroscopies to assess possible differences between human pluripotent (embryonic) and multipotent (adult mesenchymal) stem cells, and how O2 concentration in cell culture could affect the spectral signatures of these cells. Our work shows that infrared spectroscopy of embryonic (pluripotent) and adult mesenchymal (multipotent) stem cells have different spectral signatures based on the amount of lipids in their cytoplasm (confirmed with cytological staining). Furthermore, O2 concentration in cell culture causes changes in both the FTIR and Raman spectra of embryonic stem cells. These results show that embryonic stem cells might be more sensitive to O2 concentration when compared to mesenchymal stem cells. While vibrational spectroscopy could therefore be of potential use in identifying different populations of stem cells further work is required to better understand these differences.

Graphical abstract: Vibrational spectroscopy differentiates between multipotent and pluripotent stem cells

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Publication details

The article was received on 16 Jul 2010, accepted on 05 Oct 2010 and first published on 18 Oct 2010


Article type: Paper
DOI: 10.1039/C0AN00525H
Citation: Analyst, 2010,135, 3126-3132
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    Vibrational spectroscopy differentiates between multipotent and pluripotent stem cells

    J. K. Pijanka, D. Kumar, T. Dale, I. Yousef, G. Parkes, V. Untereiner, Y. Yang, P. Dumas, D. Collins, M. Manfait, G. D. Sockalingum, N. R. Forsyth and J. Sulé-Suso, Analyst, 2010, 135, 3126
    DOI: 10.1039/C0AN00525H

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