Jump to main content
Jump to site search

Issue 6, 2010
Previous Article Next Article

Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes

Author affiliations

Abstract

A microfluidic-based system was developed for the in situ monitoring of the 7-ethoxyresorufin O-dealkylation (EROD) activity of primary rat hepatocytes by measuring the fluorescent intensity of both cells and their surrounding media. The microfluidic chip was designed to allow the cell suspension and test reagent to be introduced in a layer-by-layer flow format, thereby resulting in a short mixing time by diffusion. A good linear relationship was obtained between the resorufin concentration up to 30 μM and fluorescent intensity over the chip's circular chamber area. The EROD activity was determined with 3-methylcholanthrene (3-MC)-induced hepatocytes. The inhibition effect of α-naphthoflavone was also examined on EROD activity resulting in an IC50 value of 12.98 μM.

Graphical abstract: Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes

Back to tab navigation

Publication details

The article was received on 24 Jan 2010, accepted on 19 Mar 2010 and first published on 17 Apr 2010


Article type: Paper
DOI: 10.1039/C0AN00031K
Citation: Analyst, 2010,135, 1282-1287
  •   Request permissions

    Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes

    K. Anderson, J. M. Cooper, S. J. Haswell, D. Marshall, H. Yin and X. Zhang, Analyst, 2010, 135, 1282
    DOI: 10.1039/C0AN00031K

Search articles by author

Spotlight

Advertisements