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Issue 6, 2010
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Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

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Abstract

Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus, the neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of ultraviolet radiation on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milliseconds. Chemical changes within the sub-cellular structure of the sperm cells were recorded as a function of ultraviolet light exposure time, showing the proof-of-principle that Raman microspectroscopy can be a fast diagnostic method for detecting the mitochondrial and motility status of human spermatozoa.

Graphical abstract: Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

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Publication details

The article was received on 22 Dec 2009, accepted on 24 Mar 2010 and first published on 13 Apr 2010


Article type: Paper
DOI: 10.1039/B927012D
Citation: Analyst, 2010,135, 1370-1374
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    Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

    K. Meister, D. A. Schmidt, E. Bründermann and M. Havenith, Analyst, 2010, 135, 1370
    DOI: 10.1039/B927012D

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