Jump to main content
Jump to site search

Issue 4, 2010
Previous Article Next Article

Direct analysis of lipids and small metabolites in mouse brain tissue by AP IR-MALDI and reactive LAESI mass spectrometry

Author affiliations

Abstract

Ambient analysis of metabolites and lipids from unprocessed animal tissue by mass spectrometry remains a challenge. The utility of the two novel ambient ionization techniques – atmospheric pressure infrared matrix-assisted laser desorption ionization (AP IR-MALDI) and laser ablation electrospray ionization (LAESI) – is demonstrated for the direct mass spectrometric analysis of lipids and other metabolites from mouse brain. Major brain lipids including cholesterol, various phospholipid species (glycerophosphocholines, sphingomyelin and phosphatidylethanolamines) along with numerous metabolites, for example γ-aminobutyric acid (GABA), creatine and choline, were identified in a typical mass spectrum. In a new ionization modality of LAESI, termed reactive LAESI, in-plume reactions with a solute of choice (lithium sulfate) enhanced structure-specific fragmentation of lipid ions for improved molecular assignment in collision-activated dissociation experiments. In-plume processes in reactive LAESI provide additional structural information without contaminating the biological sample with the reactant.

Graphical abstract: Direct analysis of lipids and small metabolites in mouse brain tissue by AP IR-MALDI and reactive LAESI mass spectrometry

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Nov 2009, accepted on 07 Jan 2010 and first published on 20 Jan 2010


Article type: Paper
DOI: 10.1039/B922854C
Citation: Analyst, 2010,135, 751-758
  •   Request permissions

    Direct analysis of lipids and small metabolites in mouse brain tissue by AP IR-MALDI and reactive LAESI mass spectrometry

    B. Shrestha, P. Nemes, J. Nazarian, Y. Hathout, E. P. Hoffman and A. Vertes, Analyst, 2010, 135, 751
    DOI: 10.1039/B922854C

Search articles by author

Spotlight

Advertisements