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Issue 5, 2010
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An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy

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Abstract

Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression.

Graphical abstract: An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy

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Publication details

The article was received on 30 Sep 2009, accepted on 10 Feb 2010 and first published on 04 Mar 2010


Article type: Paper
DOI: 10.1039/B920385K
Citation: Analyst, 2010,135, 887-894
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    An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy

    M. J. Baker, C. Clarke, D. Démoulin, J. M. Nicholson, F. M. Lyng, H. J. Byrne, C. A. Hart, M. D. Brown, N. W. Clarke and P. Gardner, Analyst, 2010, 135, 887
    DOI: 10.1039/B920385K

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