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Issue 3, 2010
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Fluorescence anisotropy: from single molecules to live cells

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Abstract

The polarization of light emitted by fluorescent probes is an easily accessible physical quantity that is related to a multitude of molecular parameters including conformation, orientation, size and the nanoscale environment conditions, such as dynamic viscosity and temperature. In analytical biochemistry and analytical chemistry applied to biological problems, fluorescence anisotropy is widely used for measuring the folding state of proteins and nucleic acids, and the affinity constant of ligands through titration experiments. The emphasis of this review is on new multi-parameter single-molecule detection schemes and their bioanalytical applications, and on the use of ensemble polarization assays to study binding and conformational dynamics of proteins and aptamers and for high-throughput discovery of small-molecule drugs.

Graphical abstract: Fluorescence anisotropy: from single molecules to live cells

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Publication details

The article was received on 29 Sep 2009, accepted on 09 Dec 2009 and first published on 07 Jan 2010


Article type: Minireview
DOI: 10.1039/B920242K
Citation: Analyst, 2010,135, 452-459
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    Fluorescence anisotropy: from single molecules to live cells

    C. C. Gradinaru, D. O. Marushchak, M. Samim and U. J. Krull, Analyst, 2010, 135, 452
    DOI: 10.1039/B920242K

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