Jump to main content
Jump to site search

Issue 7, 2010
Previous Article Next Article

Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

Author affiliations

Abstract

Amplified potentiometric transduction of DNA hybridization based on using liposome ‘nanocarriers’ loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced release and highly-sensitive measurements of the calcium signaling ions using a Ca2+ ion-selective electrode (ISE). The high loading yield of nearly one million signaling ions per liposome leads to sub-fmol DNA detection limits. Factors affecting the ion encapsulation efficiency and signal amplification are evaluated and discussed. The influence of the surfactant lysing agent is also examined. Such use of ‘green’ calcium signaling ions addresses the inherent toxicity of Ag and CdS nanoparticle tags used in previous potentiometric bioassays. The new strategy was applied for the detection of low levels of E. coli bacteria. It could be readily extended to trace measurements of other important biomolecules in connection to different biorecognition events. The attractive analytical performance makes liposomes a useful addition to the armory of potentiometric bioassays.

Graphical abstract: Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 02 Apr 2010, accepted on 17 May 2010 and first published on 28 May 2010


Article type: Paper
DOI: 10.1039/C0AN00198H
Citation: Analyst, 2010,135, 1618-1623
  •   Request permissions

    Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

    K. Y. Chumbimuni-Torres, J. Wu, C. Clawson, M. Galik, A. Walter, G. Flechsig, E. Bakker, L. Zhang and J. Wang, Analyst, 2010, 135, 1618
    DOI: 10.1039/C0AN00198H

Search articles by author