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Issue 2, 2010
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Resonant Mie Scattering (RMieS) correction of infrared spectra from highly scattering biological samples

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Abstract

Infrared spectra of single biological cells often exhibit the ‘dispersion artefact’ observed as a sharp decrease in intensity on the high wavenumber side of absorption bands, in particular the Amide I band at ∼1655 cm−1, causing a downward shift of the true peak position. The presence of this effect makes any biochemical interpretation of the spectra unreliable. Recent theory has shed light on the origins of the ‘dispersion artefact’ which has been attributed to resonant Mie scattering (RMieS). In this paper a preliminary algorithm for correcting RMieS is presented and evaluated using simulated data. Results show that the ‘dispersion artefact’ appears to be removed; however, the correction is not perfect. An iterative approach was subsequently implemented whereby the reference spectrum is improved after each iteration, resulting in a more accurate correction. Consequently the corrected spectra become increasingly more representative of the pure absorbance spectra. Using this correction method reliable peak positions can be obtained.

Graphical abstract: Resonant Mie Scattering (RMieS) correction of infrared spectra from highly scattering biological samples

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Publication details

The article was received on 07 Oct 2009, accepted on 30 Nov 2009 and first published on 15 Dec 2009


Article type: Paper
DOI: 10.1039/B921056C
Citation: Analyst, 2010,135, 268-277
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    Resonant Mie Scattering (RMieS) correction of infrared spectra from highly scattering biological samples

    P. Bassan, A. Kohler, H. Martens, J. Lee, H. J. Byrne, P. Dumas, E. Gazi, M. Brown, N. Clarke and P. Gardner, Analyst, 2010, 135, 268
    DOI: 10.1039/B921056C

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