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Issue 1, 2010
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Analytical protocol for identification of BMAA and DAB in biological samples

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Abstract

β-N-methylamino-L-alanine (BMAA) is a non-protein amino acid, thought to be inflicting neurodegenerative diseases related to ALS/PDC in human beings. Due to conflicting data concerning the presence of BMAA in various biological matrixes, we present a robust and sensitive method for high confidence identification of BMAA after derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The efficient sample pretreatment in combination with LC-MS/MS SRM enables chromatographic separation of BMAA from the isomer 2,3-diaminobutyric acid (DAB). The method is applicable for selective BMAA/DAB detection in various biological samples ranging from a prokaryotic cyanobacterium to eukaryotic fish.

Graphical abstract: Analytical protocol for identification of BMAA and DAB in biological samples

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Publication details

The article was received on 07 Oct 2009, accepted on 16 Nov 2009 and first published on 26 Nov 2009


Article type: Paper
DOI: 10.1039/B921048B
Citation: Analyst, 2010,135, 127-132
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    Analytical protocol for identification of BMAA and DAB in biological samples

    Z. Spáčil, J. Eriksson, S. Jonasson, U. Rasmussen, L. L. Ilag and B. Bergman, Analyst, 2010, 135, 127
    DOI: 10.1039/B921048B

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