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Issue 2, 2010
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Highly sensitive protein detection using enzyme-labeled gold nanoparticle probes

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Abstract

A highly sensitive protein detection method based on a novel enzyme-labeled gold nanoparticle (AuNP) probe has been developed. In this method, we firstly prepared the enzyme-labeled AuNP probe by coating AuNP with antibody, single-stranded DNA (ssDNA), and horseradish peroxidase (HRP). Magnetic microparticle (MMP) functionalized with another antibody was used as capture probe. Then, target protein was sandwiched by the enzyme-labeled AuNP probe and the capture probe through immunoreaction. The target immunoreaction event could be sensitively transduced via the enzymatically amplified optical signal. By using this strategy, carcinoembryonic antigen (CEA), as a model protein, was detected with high sensitivity and good specificity. The detection limit for this approach was 12 ng Lāˆ’1, which was approximately 130-fold more sensitive than the conventional enzyme-linked immunosorbent assay (ELISA). The practical application of the proposed immunoassay was carried out for determination of CEA in serum samples. The demonstrated capability of the proposed method shows potentially applications for early diagnoses of diseases.

Graphical abstract: Highly sensitive protein detection using enzyme-labeled gold nanoparticle probes

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Publication details

The article was received on 12 Aug 2009, accepted on 01 Dec 2009 and first published on 10 Dec 2009


Article type: Paper
DOI: 10.1039/B916629G
Citation: Analyst, 2010,135, 327-331
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    Highly sensitive protein detection using enzyme-labeled gold nanoparticle probes

    M. Liu, C. Jia, Y. Huang, X. Lou, S. Yao, Q. Jin, J. Zhao and J. Xiang, Analyst, 2010, 135, 327
    DOI: 10.1039/B916629G

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