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Issue 6, 2009
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Micropatterning of bioactive self-assembling gels

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Abstract

Microscale topographical features have been known to affect cell behavior. An important target in this area is to integrate top down techniques with bottom up self-assembly to create three-dimensional (3D) patterned bioactive mimics of extracellular matrices. We report a novel approach toward this goal and demonstrate its use to study the behavior of human mesenchymal stem cells (hMSCs). By incorporating polymerizable acetylene groups in the hydrophobic segment of peptide amphiphiles (PAs), we were able to micro-pattern nanofiber gels of these bioactive materials. PAs containing the cell adhesive epitope arginineglycineaspartic acidserine (RGDS) were allowed to self-assemble within microfabricated molds to create networks of either randomly oriented or aligned ∼30 nm diameter nanofiber bundles that were shaped into topographical patterns containing holes, posts, or channels up to 8 µm in height and down to 5 µm in lateral dimensions. When topographical patterns contained nanofibers aligned through flow prior to gelation, the majority of hMSCs aligned in the direction of the nanofibers even in the presence of hole microtextures and more than a third of them maintained this alignment when encountering perpendicular channel microtextures. Interestingly, in topographical patterns with randomly oriented nanofibers, osteoblastic differentiation was enhanced on hole microtextures compared to all other surfaces.

Graphical abstract: Micropatterning of bioactive self-assembling gels

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Publication details

The article was received on 27 Oct 2008, accepted on 19 Dec 2008, published on 23 Feb 2009 and first published online on 23 Feb 2009


Article type: Paper
DOI: 10.1039/B819002J
Citation: Soft Matter, 2009,5, 1228-1236
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    Micropatterning of bioactive self-assembling gels

    A. Mata, L. Hsu, R. Capito, C. Aparicio, K. Henrikson and S. I. Stupp, Soft Matter, 2009, 5, 1228
    DOI: 10.1039/B819002J

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