Photosensitized production of singlet oxygen: spatially-resolved optical studies in single cells

Thomas Breitenbach; Marina K. Kuimova; Peter Gbur; Sonja Hatz; Nickolass Bitsch Schack; Brian Wett Pedersen; John D. C. Lambert; Lars Poulsen; Peter R. Ogilby

doi:10.1039/B809049A

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Photochemical & Photobiological Sciences

Issue 4, 2009

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Photosensitized production of singlet oxygen: spatially-resolved optical studies in single cells

Thomas Breitenbach , Marina K. Kuimova , Peter Gbur , Sonja Hatz , Nickolass Bitsch Schack , Brian Wett Pedersen , John D. C. Lambert , Lars Poulsen and Peter R. Ogilby

Photochem. Photobiol. Sci., 2009, 8, 442-452

DOI: 10.1039/B809049A
Received 30 May 2008, Accepted 04 Aug 2008
First published on the web 23 Sep 2008
This article is part of the collection: Microscopy beyond imaging: space-resolved photochemistry and photobiology

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Singlet molecular oxygen, O₂(a¹Δ_g), can be created in photosensitized experiments with sub-cellular spatial resolution in a single cell. This cytotoxic species can subsequently be detected by its 1270 nm phosphorescence (a¹Δ_g→ X³Σ⁻_g). Cellular responses to the creation of singlet oxygen can be monitored using viability assays. Time- and spatially-resolved optical measurements of both singlet oxygen and its precursor, the excited state sensitizer, reflect the complex and dynamic morphology of the cell. These experiments help elucidate photoinduced, oxygen-dependent events that compromise cell function and ultimately lead to cell death. In this perspective, recent work on the photosensitized production and detection of singlet oxygen in single cells is summarized, highlighting the advantages and current limitations of this unique experimental approach to study an old problem.

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Thomas Breitenbach
Marina K. Kuimova
Peter Gbur
Sonja Hatz
Nickolass Bitsch Schack
Brian Wett Pedersen
John D. C. Lambert
Lars Poulsen
Peter R. Ogilby

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Photochemical & Photobiological Sciences

Photosensitized production of singlet oxygen: spatially-resolved optical studies in single cells

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