Issue 19, 2009
From the journal:

Organic & Biomolecular Chemistry

Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases†

Yajun Yan,a   Chia-Chi Leea  and  James C. Liao*a  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531, Boelter Hall, 420 Westwood Plaza, Los Angeles, CA, USA
E-mail: Liaoj@ucla.edu
Fax: 310-206-4107
Tel: 310-205-1656

Abstract

We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).

Graphical abstract: Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

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Article information

DOI
https://doi.org/10.1039/B913501D
Article type
Communication
Submitted
07 Jul 2009
Accepted
23 Jul 2009
First published
03 Aug 2009

Org. Biomol. Chem., 2009,7, 3914-3917

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Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

Y. Yan, C. Lee and J. C. Liao, Org. Biomol. Chem., 2009, 7, 3914 DOI: 10.1039/B913501D

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