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Issue 19, 2009
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Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

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Abstract

We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).

Graphical abstract: Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

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Publication details

The article was received on 07 Jul 2009, accepted on 23 Jul 2009 and first published on 03 Aug 2009


Article type: Communication
DOI: 10.1039/B913501D
Citation: Org. Biomol. Chem., 2009,7, 3914-3917
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    Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

    Y. Yan, C. Lee and J. C. Liao, Org. Biomol. Chem., 2009, 7, 3914
    DOI: 10.1039/B913501D

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