The effect of microwave irradiation on DNA hybridization

Wesleigh F. Edwards; Douglas D. Young; Alexander Deiters

doi:10.1039/B903609A

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Organic & Biomolecular Chemistry

Issue 12, 2009

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The effect of microwave irradiation on DNA hybridization

Wesleigh F. Edwards , Douglas D. Young and Alexander Deiters

Org. Biomol. Chem., 2009, 7, 2506-2508

DOI: 10.1039/B903609A
Received 20 Feb 2009, Accepted 17 Apr 2009
First published on the web 01 May 2009

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Abstract
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The effect of microwave irradiation on DNA/DNA hybridization has been studied under controlled power and temperature conditions. It was discovered that microwave irradiation led to the melting of double-stranded deoxyoligonucleotides well below their thermal melting temperature and independent of the length of the deoxyoligonucleotides. These observations indicate a specific interaction of microwaves with DNA, and have important implications in the chemical or enzymatic processing of DNA under microwave heating.

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Hybridization and melting protocols under thermal andmicrowave conditions; power/temperature/pressure profiles ofmicrowave experiments
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Organic & Biomolecular Chemistry

The effect of microwave irradiation on DNA hybridization

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