Carbosilane        dendrimers peripherally functionalized with dansyl fluorescence tags and their cellular internalization studies

Paula Ortega; Ma Jesús Serramía; Rafael Samaniego; F. Javier de la Mata; Rafael Gomez; Ma Angeles Muñoz-Fernandez

doi:10.1039/B900942F

Carbosilane dendrimers peripherally functionalized with dansyl fluorescence tags and their cellular internalization studies

Paula Ortega,^a^b Ma Jesús Serramía,^b^c Rafael Samaniego,^d F. Javier de la Mata,*^a^b Rafael Gomez*^a^b and Ma Angeles Muñoz-Fernandez*^b^c

Author affiliations

* Corresponding authors

^a Departamento de Química Inorgánica, Universidad de Alcalá, Campus Universitario, E-28871 Alcalá de Henares, Spain
E-mail: rafael.gomez@uah.es
Fax: (+34) 91 885 4683

^b Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain

^c Laboratorio de Inmunobiología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain
E-mail: mmunoz@cbm.uam.es

^d Unidad de Microscopía Confocal, Hospital General Universitario Gregorio Marañón, Madrid, Spain

Abstract

This paper describes the synthesis of carbosilane dendrimers containing dansyl groups at their periphery showing two topologies: as per-dansylated systems of type G_n-[Si(CH₂)₃NH(Dans)]_m or as random partially surface-modified carbosilane dendrimers containing both amine and dansyl groups G₂-{[Si(CH₂)₃NH₂]_x[Si(CH₂)₃NH(Dans)]_y}. The dendritic systems were tested for in vitro toxicity on primary cell cultures (dendritic and peripheral blood mononuclear cells) and their cellular uptake was studied using confocal fluorescence microscopy. The results suggest that both types of dendritic topologies are acting as fluorescent labels proving the internalization of the carbosilane skeleton into cells.

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Publication details

The article was received on 16 Jan 2009, accepted on 06 May 2009 and first published on 09 Jun 2009

Article type: Paper

DOI: 10.1039/B900942F

Citation: Org. Biomol. Chem., 2009,7, 3079-3085

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Carbosilane dendrimers peripherally functionalized with dansyl fluorescence tags and their cellular internalization studies

P. Ortega, M. xmlns="http://www.rsc.org/schema/rscart38"> <. J. Serramía, R. Samaniego, F. J. de la Mata, R. Gomez and M. xmlns="http://www.rsc.org/schema/rscart38"> <. A. Muñoz-Fernandez, Org. Biomol. Chem., 2009, 7, 3079
DOI: 10.1039/B900942F
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Organic & Biomolecular Chemistry

Carbosilane dendrimers peripherally functionalized with dansyl fluorescence tags and their cellular internalization studies

Abstract

Supplementary files

Publication details

Carbosilane dendrimers peripherally functionalized with dansyl fluorescence tags and their cellular internalization studies

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