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Issue 5, 2009
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One- and two-photon activated phototoxicity of conjugated porphyrin dimers with high two-photon absorption cross sections

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Abstract

Two-photon excited photodynamic therapy (PDT) has the potential to provide a highly targeted treatment for neoplastic diseases, as excitation can be pin-pointed to small volumes at the laser focus. In addition, two-photon PDT offers deeper penetration into mammalian tissue due to the longer wavelength of irradiation. Here we report the one-photon and two-photon excited PDT results for a collection of conjugated porphyrin dimers with high two-photon absorption cross sections. These dimers demonstrate high one-photon PDT efficacy against a human ovarian adenocarcinoma cell line (SK-OV-3) and exhibit no significant dark-toxicity at concentrations of up to 20 μM. Their one-photon excited PDT efficiencies, following irradiation at 657 nm, approach that of Visudyne®, a drug used clinically for PDT. We investigated and optimised the effect of the photosensitiser concentration, incubation time and the light dose on the PDT efficacy of these dimers. These studies led to the selection of P2C2-NMeI as the most effective porphyrin dimer. We have demonstrated that P2C2-NMeI undergoes a two-photon activated process following excitation at 920 nm (3.6–6.8 mW, 300 fs, 90 MHz) and compared it to Visudyne. We conclude that the in vitro two-photon PDT efficacy of P2C2-NMeI is about twice that of Visudyne. This result highlights the potential of this series of porphyrin dimers for two-photon PDT.

Graphical abstract: One- and two-photon activated phototoxicity of conjugated porphyrin dimers with high two-photon absorption cross sections

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Publication details

The article was received on 27 Aug 2008, accepted on 10 Nov 2008 and first published on 09 Jan 2009


Article type: Paper
DOI: 10.1039/B814792B
Citation: Org. Biomol. Chem., 2009,7, 897-904
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    One- and two-photon activated phototoxicity of conjugated porphyrin dimers with high two-photon absorption cross sections

    E. Dahlstedt, H. A. Collins, M. Balaz, M. K. Kuimova, M. Khurana, B. C. Wilson, D. Phillips and H. L. Anderson, Org. Biomol. Chem., 2009, 7, 897
    DOI: 10.1039/B814792B

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