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Issue 2, 2009
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Polycationic phosphorus dendrimers: synthesis, characterization, study of cytotoxicity, complexation of DNA, and transfection experiments

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Abstract

Four series of phosphorus dendrimers (generations 1 and 4) having various types of amine terminal groups (pyrrolidine, morpholine, methyl piperazine, or phenyl piperazine) are synthesized. After protonation, the fourth generations of three of them are found water-soluble, and used for several biological experiments. First, the cytotoxicity of these polycationic dendrimers towards three cell strains (one healthy: HUVEC, and two cancerous: HEK 293 and HeLa) is assayed and found low. Second, their ability to interact with DNA is tested by electrophoresis: the dendrimer terminated by pyrrolidinium groups is found efficient to form dendriplexes. Finally, the polycationic dendrimers are used as transfection agents to deliver single- and double-stranded DNA into the three above-mentioned cell strains. Here also the dendrimer having pyrrolidinium groups is found the most efficient.

Graphical abstract: Polycationic phosphorus dendrimers: synthesis, characterization, study of cytotoxicity, complexation of DNA, and transfection experiments

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Publication details

The article was received on 02 Sep 2008, accepted on 10 Oct 2008 and first published on 26 Nov 2008


Article type: Paper
DOI: 10.1039/B815259D
Citation: New J. Chem., 2009,33, 318-326
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    Polycationic phosphorus dendrimers: synthesis, characterization, study of cytotoxicity, complexation of DNA, and transfection experiments

    C. Padié, M. Maszewska, K. Majchrzak, B. Nawrot, A. Caminade and J. Majoral, New J. Chem., 2009, 33, 318
    DOI: 10.1039/B815259D

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