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Issue 2, 2009
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Synchrotron radiation for direct analysis of metalloproteins on electrophoresis gels

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Abstract

Metalloproteomics requires analytical techniques able to assess and quantify the inorganic species in metalloproteins. The most widely used methods are hyphenated techniques, based on the coupling of a high resolution chromatographic method with a high sensitivity method for metal analysis in solution. An alternative approach is the use of methods for solid sample analysis, combining metalloprotein separation by gel electrophoresis and direct analysis of the gels. Direct methods are based on beam analysis, such as lasers, ion beams or synchrotron radiation beams. The aim of this review article is to present the main features of synchrotron radiation based methods and their applications for metalloprotein analysis directly on electrophoresis gels. Synchrotron radiation X-ray fluorescence has been successfully employed for sensitive metal identification, and X-ray absorption spectroscopy for metal local structure speciation in proteins. Synchrotron based methods will be compared to ion beam and mass spectrometry for direct analysis of metalloproteins in electrophoresis gels.

Graphical abstract: Synchrotron radiation for direct analysis of metalloproteins on electrophoresis gels

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Publication details

The article was received on 11 Nov 2008, accepted on 05 Feb 2009 and first published on 24 Feb 2009


Article type: Minireview
DOI: 10.1039/B820150C
Citation: Metallomics, 2009,1, 137-141
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    Synchrotron radiation for direct analysis of metalloproteins on electrophoresis gels

    R. Ortega, Metallomics, 2009, 1, 137
    DOI: 10.1039/B820150C

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