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Issue 12, 2009
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Analysis of structured and intrinsically disordered regions of transmembraneproteins

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Abstract

Integral membrane proteins display two major types of transmembrane structure, helical bundles and beta barrels. The main functional roles of transmembraneproteins are the transport of small molecules and cell signaling, and sometimes these two roles are coupled. For cytosolic, water-soluble proteins, signaling and regulatory functions are often carried out by intrinsically disordered regions. Our long range goal is to determine whether integral membrane proteins likewise use disordered regions for signaling and regulation. Here we carried out a systematic bioinformatics investigation of intrinsically disordered regions obtained from integral membrane proteins for which crystal structures have been determined, and for which the intrinsic disorder was identified as missing electron density. We found 120 disorder-containing integral membrane proteins having a total of 33 675 residues, with 3209 of the residues distributed among 240 different disordered regions. These disordered regions were compared with those obtained from water-soluble proteins with regards to their amino acid compositional biases, and to the accuracies of various disorder predictors. The results of these analyses show that the disordered regions from helical bundle integral membrane proteins, those from beta barrel integral membrane proteins, and those from water soluble proteins all exhibit statistically distinct amino acid compositional biases. Despite these differences in composition, current algorithms make reasonably accurate predictions of disorder for these membrane proteins. Although the small size of the current data sets are limiting, these results suggest that developing new predictors that make use of data from disordered regions in helical bundles and beta barrels, especially as these datasets increase in size, will likely lead to significantly more accurate disorder predictions for these two classes of integral membrane proteins.

Graphical abstract: Analysis of structured and intrinsically disordered regions of transmembraneproteins

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Publication details

The article was received on 24 Mar 2009, accepted on 15 Apr 2009 and first published on 01 Jun 2009


Article type: Paper
DOI: 10.1039/B905913J
Citation: Mol. BioSyst., 2009,5, 1688-1702
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    Analysis of structured and intrinsically disordered regions of transmembraneproteins

    B. Xue, L. Li, S. O. Meroueh, V. N. Uversky and A. K. Dunker, Mol. BioSyst., 2009, 5, 1688
    DOI: 10.1039/B905913J

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