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Issue 12, 2009
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A global view of Escherichia coli Rsd protein and its interactions

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Abstract

The Escherichia coli Rsd protein forms 1 : 1 complexes with σ70protein, which is the major factor in determining promoter recognition by RNA polymerase. Here we describe measurements of the levels of Rsd, RNA polymerase, σ70 and the alternative σ38 factor. Rsd levels are sufficient to sequester a significant proportion of σ70 and immunoaffinity pull-down experiments show that this occurs in stationary phase but not in exponentially growing cells. Rsd expression is controlled by two promoters, P1 and P2. Experiments with lac fusions show that the P2 promoter is stronger than P1, that P2 is active in all phases of growth, and that this accounts for the high levels of Rsd.

Graphical abstract: A global view of Escherichia coli Rsd protein and its interactions

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Publication details

The article was received on 11 Mar 2009, accepted on 13 Aug 2009 and first published on 14 Sep 2009


Article type: Paper
DOI: 10.1039/B904955J
Citation: Mol. BioSyst., 2009,5, 1943-1947
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    A global view of Escherichia coli Rsd protein and its interactions

    S. E. Piper, J. E. Mitchell, D. J. Lee and S. J. W. Busby, Mol. BioSyst., 2009, 5, 1943
    DOI: 10.1039/B904955J

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