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Issue 10, 2009
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Systems biology meets synthetic biology: a case study of the metabolic effects of synthetic rewiring

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Abstract

We present a systems biology approach to study the global metabolic effects of the insertion of synthetic circuits in a cellular chassis. Our approach combines high-throughput proteomics with the MMG probabilistic tool, which integrates the data with the metabolic circuit’s topology. We present a theoretical analysis of the foundations of our approach, as well as experimental results on a mutant strain of Escherichia coli where a light-receptor circuit was inserted and coupled with lactose metabolism. Our results show that the systems approach manages to extract meaningful information from the proteomic data that cannot be recovered by naive thresholding of the data. This tool can be used to characterise the relationship between new circuits and chassis in synthetic biology applications.

Graphical abstract: Systems biology meets synthetic biology: a case study of the metabolic effects of synthetic rewiring

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Publication details

The article was received on 09 Mar 2009, accepted on 28 May 2009 and first published on 07 Jul 2009


Article type: Paper
DOI: 10.1039/B904729H
Citation: Mol. BioSyst., 2009,5, 1214-1223
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    Systems biology meets synthetic biology: a case study of the metabolic effects of synthetic rewiring

    J. Noirel, S. Y. Ow, G. Sanguinetti and P. C. Wright, Mol. BioSyst., 2009, 5, 1214
    DOI: 10.1039/B904729H

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